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. 2015 Sep 9;10(9):e0137170. doi: 10.1371/journal.pone.0137170

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© 2015 Tornero-Esteban et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — a) In vitro differentiation and characterization of MSCs. Cultured MSCs from two healthy donors and two OA donors showing similar, plate adherence and fibroblast-like morphology at passage 3. Growth characteristics of MSCs from OA donors were similar as well as the degree of mineralization during osteogenic lineage commitment revealed by Alizarin red S staining (Panel A). (Magnification x 50). b) quantitative measurement of alizarin red staining of osteogenic cultures.