Fig 4. Electrophoretic Mobility Shift Assay (EMSA) PAGE of MalE-VirF Binding to pvirB DNA Probe in Presence of Small Molecule Hit Compounds.

EMSA image shows the retardation of the 5’Cy5-pvirB DNA probe (0.25 μM) when incubated in the presence of MalE-VirF (1 μM). Image also shows the effect each of the five hit compounds have on MalE-VirF binding. All compounds were dosed at 100 μM, and only one compound, 19615, appeared to inhibit MalE-VirF binding to the pvirB DNA probe.