Fig 7. Characterization of Hit Compound 19615.

A) Plot of inhibition of MalE-VirF binding to the pvirB DNA probe by 19615. Assayed as in Fig 5 with the MalE-VirF concentration constant at 20 μM. B) Parameters for 19615. An IC₅₀ of 46 ± 2.2 μM for DNA binding was determined, which, by the Cheng-Prusoff equation, yields a derived K _i = 5.6 μM. The assay was conducted in duplicate. * Data from Emanuele et al. J. Antibiotics 67:379–386 (2014).