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. 2015 Sep 9;10(9):e0137504. doi: 10.1371/journal.pone.0137504

Fig 1. Transfection efficiency in human bronchial epithelia cells (16HBE)–luciferase activity.

Fig 1

Lipo- and polyplexes were prepared as described in the material and methods section. A fraction of the complexes was kept apart and used as a non-nebulised control (white bars). The rest of the solution was aerosolized for 5 minutes by employing a PARI Boy® Nebulizer (black bars) and collected. Nebulised and non-nebulised complexes were incubated with 16HBE cells for 2 h. Luciferase activity in 50 μl of supernatants was assayed after 24 h and its activity is expressed in relative light units, n ≥ 6. The average relative light units have been employed to evaluate differences between samples. 100 ng of mRNA complexed with 1.2 μl of Lipofectamine or 1.5 μl DMRIE or 0.8 μl linPEI or 0.9 μl brPEI (500 ng) were added per well; **p < 0.001.