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. 2015 Sep 9;10(9):e0137504. doi: 10.1371/journal.pone.0137504

Fig 2. Transfection efficiency in human bronchial epithelia cells—numbers of GFP positive cells.

Fig 2

Lipoplexes were prepared in OptiMem. A fraction of the complexes was kept apart and used as a non-nebulised control. The rest of the solution was aerosolized for 5 minutes by employing a PARI Boy® Nebulizer and collected. Nebulised and non-nebulised complexes were incubated with 16HBE cells for 2 h. 100 ng of IVT mRNA complexed with 1.2 μl of Lipofectamine or 1.5 μl of DMRIE were added per well. For brPEI 500 ng IVT mRNA complexed with 0.9 μl brPEI were used. The numbers of GFP positive cells were determined by flow cytometric analysis and by acquiring pictures at a fluorescent microscope. A-B non-nebulised Lipofectamine-mGFP complexes; C-D nebulised Lipofectamine-mGFP complexes; E—numbers of GFP-positive cells achieved by nebulised and non-nebulised Lipofectamine-GFP complexes evaluated by flow cytometry (n = 9); F-G not nebulised DMRIE-mGFP complexes; H-I nebulised DMRIE-mGFP complexes.