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. 2015 Sep 9;10(9):e0136696. doi: 10.1371/journal.pone.0136696

Fig 1. Experimental design.

Fig 1

Forty crossbred females calves aged between 4 to 8 months were divided in two experimental groups: group S19—composed of 20 calves vaccinated with S19 vaccine strain (0.6–1.2 x 1011 CFU) at day 0 of the experiment; and group RB51—composed of 20 calves vaccinated with RB51 vaccine strain (1.3 x 1010 CFU) at day 0 of the experiment. Both groups were revaccinated with RB51 (1.3 x 1010 CFU) at day 365 of the experiment. The number of animals tested in each immunological assessment (0,28, 210, 365, 393 and 575) are shown in the rectangles. The days when the vaccinations occurred are highlighted with arrows.