Table 1. Monoclonal antibodies (mAbs) against cell surface markers, intracellular cytokine, nuclear protein or immunoglobulin used in this study.

mAb	Conjugated	Target species	Clone	Host	Isotype	Binding site	Concentration or dilution
Anti-CD4 a	Alexa Fluor 647 / FITC g / PE h	Bovine	CC8	Mouse	IgG2a	Cell surface	0.25 to 0.5 μg/mL
Anti-CD8 a	Alexa Fluor 647 / FITC / PE	Bovine	CC63	Mouse	IgG2a	Cell surface	0.25 to 0.5 μg/mL
Anti-CD21 a	FITC / PE	Bovine	CC21	Mouse	IgG1	Cell surface	0.5 to 1.0 μg/mL
Anti-MHC II a	FITC	Bovine	IL-A21	Mouse	IgG2a	Cell surface	0.5 to 1.0 μg/mL
Anti-CD25 a	PE	Bovine	IL-A111	Mouse	IgG1	Cell surface	0.5 to 1.0 μg/mL
Anti-CD45 RO b	-	Bovine	GC42A1	Mouse	IgG1	Cell surface	2 to 5 μg/mL
Anti-IgG1 c	PE-Cy5.5	Mouse		Goat	IgG1	Cell surface	1: 10
Anti-FoxP3 a	Alexa Fluor 647	Bovine	7627	Human	HuCAL Fab bivalent	Intracellular	1: 25
Anti-IL-4 a	PE	Bovine	CC303	Mouse	IgG2a	Intracellular	1: 50
Anti-IFN-ɣ a	PE	Bovine	CC302	Mouse	IgG1	Intracellular	1: 50
Anti-IL-17A d	PE	Human	eBio64DEC17	Mouse	IgG1	Intracellular	0.25 to 0.5 μg/mL
Anti-Granzyme B e	PE	Human	351927	Mouse	IgG2a	Intracellular	1 μg/mL
Anti-Perforin f	PE	Human	δG9	Mouse	IgG2b	Intracellular	1: 50
Anti-Total IgG a	HRP i	Bovine	IL-A2	Mouse	IgG1	Ig	1: 5000
Anti-IgG1 a	HRP	Bovine	IL-A60	Mouse	IgG1	Ig	1: 2500
Anti-IgG2 a	HRP	Bovine	IL-A73	Mouse	IgG1	Ig	1: 2500
Anti-IL-10	Biotin	Bovine	CC320	Mouse	IgG1	IL-10	3 μg/mL
Anti-IL-10	-	Bovine	CC318	Mouse	IgG2b	IL-10	7 μg/mL

^amAb purchased from AbD Serotec (Raleigh, USA).

^bmAb purchased from VMRD (Pullman, USA).

^cmAb purchased from Life Technologies (Carlsbad, USA).

^dmAb purchased from eBioscience (San Diego, USA).

^emAb purchased from R&D Systems (Minneapolis, USA).

^fmAb purchased from BD Pharmingen (San Diego, USA).

^gfluorescein isothiocyanate (FITC).

^hphycoerythrin (PE).

ⁱhorseradish peroxidase.