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. 2015 Sep 9;10(9):e0136199. doi: 10.1371/journal.pone.0136199

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© 2015 Freeman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Hierarchal clustering (HC) of mMSCs for a set of genes related to MSC multipotency. Genes are organized either by genes expressed highly in mMSCs (red), genes expressed moderately/heterogeneously in mMSCs (pink), genes that are lowly expressed in mMSCs (blue) or housekeeping genes (green). Global Z-Score reflects the number of standard deviations away from the mean of expression in the reference. (B) Principal component analysis (PCA) analysis of single cell mMSCs (1–16) (red), negative control single cells (HL1cm1-HL1cm5) (brown), and population controls (PC and HL1cmPC). (C) Quantitative reverse transcriptase polymerase chain reaction (qPCR) confirmation of RNA-seq data.