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. 2015 Sep 9;10(9):e0136199. doi: 10.1371/journal.pone.0136199

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© 2015 Freeman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — (A) HC of single cell mMSCs and mMSC population control (mMSC-PC) for a set of genes related to osteogenesis, chondrogenesis, adipogenesis, vascular smooth muscle, neurogenesis, and housekeeping genes. (B) PCA analysis of single cell mMSCs (1–16) and population control (PC). (C) PCA loading plot showing individual differentiation genes represented in (B). (D) qPCR confirmation of RNA-seq data. (E) A key transcription factor was selected for each differentiation lineage and expression was normalized to Gapdh for each sample.