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. 2015 Sep 9;10(9):e0137211. doi: 10.1371/journal.pone.0137211

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© 2015 Yao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Diagram showing the sites on the A–P axis at which iNSCs were transplanted and the migration pathway of the transplanted iNSCs (green dots) in the lesion (shaded area). (B) One day post-transplantation, iNSCs with GFP had assembled at the injection site. (C) Migrated graft cells expressing the GFP marker across the lesion of MCAO rats 6 weeks after transplantation. (D) Higher magnification of demarked area in C. Migrated cells survived in the lesion of the right cerebral cortex. Scale bar = 50 μm. (E) Confocal images: Graft cells labeled with GFP are green, nuclei stained with DAPI are blue, and various phenotypic markers are in red. iNSC1 cells differentiated into GFAP-positive astrocytes in 2 weeks, and Tuj1-positive neurons in 6 weeks. Undifferentiated iNSCs were detected by Nestin antibody. Scale bar = 25 μm.