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. 2015 Sep 9;13(9):e1002247. doi: 10.1371/journal.pbio.1002247

Fig 4. Panda belongs to a subfamily of TGF-β that includes Drosophila Maverick and vertebrate GDF15.

Fig 4

Phylogenetic analysis of TGF-β ligands using the maximum likelihood method. The analysis was performed using the full-length proteins. Representative taxa from deuterostomes, protostomes, and cnidarians were used (see S1 Text for a list of these taxa). These 162 sequences were collected from diverse databases using the National Center for Biotechnology Information (NCBI) research tool (http://www.ncbi.nlm.nih.gov/). Full-length sequences were aligned using ClustalOmega with default parameters (http://www.ebi.ac.uk/clustalw/), and gap optimisation and obvious alignment error correction were made using Bioedit 7.0.5.3 (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). The tree was calculated using the maximum likelihood method with PhyML with substitution model WAG (http://atgc.lirmm.fr/phyml/). A consensus tree with a 45% cutoff value was derived from 500 bootstrap analysis using Mega 3.1 (http://www.megasoftware.net/). Numbers above nodes represent a percentage of bootstrap values supporting this node. The original tree is presented in S5 Fig.