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. 2015 Sep 9;13(9):e1002247. doi: 10.1371/journal.pbio.1002247

Fig 5. The TGF-β ligand Panda is expressed maternally in a D/V gradient.

Fig 5

(A) RT-PCR analysis of Panda mRNA. An aliquot of the PCR reaction was run on a 1% agarose gel, and the gel was stained with Syber safe. Top panel, panda expression. Bottom panel, expression of mkk3 used as a control. Panda is expressed in ovaries and unfertilized eggs as well as during the cleavage, blastula, gastrula, and pluteus stages. (B) Expression of panda mRNA analyzed by in situ hybridization. Whole mount in situ hybridizations with panda alone or with panda (red) and nodal (blue) probes. A gradient of maternal panda mRNA is detected in immature ovocytes and to a lesser extent in the unfertilized mature eggs, whereas during the cleavage and blastula stages, panda mRNA is detected in a shallow D/V gradient. VEB, very early blastula (about 120 cells); EB, early blastula (about 220 cells); PHB, prehatching blastula (about 300 cells); HB, hatching blastula (about 400 cells); MB, mesenchyme blastula; LV, lateral view; VV, vegetal view; V, ventral; D, dorsal; FV, frontal view.