Table 1. DEVDase activity and apoptotic morphology in control and photodamaged cells.
Cells were treated with 0.3 μM AlPc for 15 min and then irradiated with 50 or 100 mJ/cm2 of red light. DEVDase activity (units = pmols/mg protein/60 min) was measured after an additional incubation for 4 or 24 h in growth medium at 37°C. Results are mean ± SD of three determinations. Dark controls were assayed 24 h after transfection. The percentage of apoptotic cells was estimated from a survey of three fields of 100 cells labeled with HO342.
| MCF10Aa
|
||||||
|---|---|---|---|---|---|---|
| 0 mJ/cm2b
|
50 mJ/cm2b
|
100 mJ/cm2b
|
||||
| DEVDase | % apoptotic | DEVDase | % apoptotic | DEVDase | % apoptotic | |
| Dark controls | 0.5 ± 0.1 | <2 | ||||
| PDT + 4 h | 1.4 ± 0.3 | <5 | 7 ± 3 | 31 ± 6 | ||
| PDT + 24 h | 26.3 ± 2.7 | 25 | 65 ± 6 | 60 ± 5 | ||
|
| ||||||
| MCF10A/bcl-2a | ||||||
|
| ||||||
| Dark controls | 0.6 ± 0.3 | <2 | ||||
| PDT + 4 h | 13 ± 3.2 | 41 ± 4 | 24 ± 5 | 75 ± 4 | ||
| PDT + 24 h | 102 ± 16 | 65 ± 7 | 110 ± 9 | 83 ± 3 | ||
|
| ||||||
| MCF10A/baxa | ||||||
|
| ||||||
| Dark controls | 25 ± 4.1 | 21 ± 4 | ||||
| PDT + 4 h | 36 ± 5.9 | 52 ± 5 | ||||
a
Cell line.
b
Light dose.