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. 2015 Sep 9;10(9):e0136893. doi: 10.1371/journal.pone.0136893

Fig 1. Acute cisplatin activates the Epidermal Growth Factor Receptor (EGFR).

Fig 1

A) Representative western blots of activated or phosphorylated EGFR (pEGFR, tyr1068) in OVCA 433 cells given 10 μM cisplatin for 0.5, 1, 2, 4 and 6 hours compared to untreated control cells. 10 nM EGF given to cells for 15 minutes as a positive control showing activation of the receptor. Total EGFR blot and GAPDH representative blots also shown. B) Graph of EGFR activation following 10 μM cisplatin treatment for 0.5, 1, 2, 4 and 6 hours, n = 6. EGF data shown as a positive control. C) Graph showing ratio of pEGFR to total EGFR (pEGFR/total EGFR ratio) following 10 μM cisplatin treatment for 0.5, 1, 2, 4 and 6 hours, n = 6. One-way ANOVA of data revealed a significant effect of cisplatin treatment and significant differences from control as determined by Dunnet’s post hoc analysis indicated by *p<0.05, **p<0.01, ***p<0.001.