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. 2015 Sep 9;10(9):e0137439. doi: 10.1371/journal.pone.0137439

Fig 4. Palindromic repeats in ERF1 promoter are not necessary for EIN3 binding.

Fig 4

DNA-binding ability of EIN3 82–352 to (A) ERF1 probes with 3’ palindromic repeat gradually deleted and (B) ERF1 probes with 5’ palindromic repeat mutated. In schematic structural diagram of ERF1, (dark) blue filled boxes and red arrow indicate palindromic repeats and EBS, respectively. (C) Comparisons of binding affinity of 36 bp probe 2EBS-S10-36 and ERF1, demonstrating that EIN3 bound tighter to the two EBS-containing 2EBS-S10-36 than ERF1.