Fig. 2.

Effect of CAL on I_Ca, LTCC expression and the systolic Ca transient.

A: Representative L-type Ca currents (I_Ca) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I_Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or GAPDH (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F₀, time to peak (TTP), and rate of rise (ΔF/F₀.ms^− 1) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ^⁎⁎⁎p < 0.001 vs Sham.