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. Author manuscript; available in PMC: 2016 Sep 25.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Aug 20;465(3):601–605. doi: 10.1016/j.bbrc.2015.08.069

Fig 2.

Fig 2

siRNA knockdown of Exoc 6 and 6b. The immunoblot for Exoc6/6b was performed with SDS samples of 3T3-L1 adipocytes containing control siRNA alone (Con), siRNA for Exoc6 or Exoc6b plus Con, or a mixture of siRNAs for Exoc 6 and 6b. The 1× load was 20 μg protein. The standards (Stds) were purified recombinant 3×Flag-tagged Exoc 6 or 6b. The 1 load for the standards was 1 ng. The 3×Flag tag on the standards decreased their mobility slightly. A replicate of this immunoblot with SDS samples from a separate experiment gave similar results.