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. Author manuscript; available in PMC: 2015 Sep 9.
Published in final edited form as: Mol Pharm. 2015 Aug 7;12(9):3237–3249. doi: 10.1021/acs.molpharmaceut.5b00183

Figure 5.

Figure 5

Tryptic peptides of Mb detected as peptide-water adducts in (A) lyophilized Mb-SDA, (B) Mb-SDA solution, (C) Mb-SDA lyophilized with raffinose, (D) Mb-SDA solution with raffinose, (E) Mb-SDA lyophilized with Gdn HCl and (F) Mb-SDA solution with Gdn HCl formulations. Tryptic peptides of Mb detected as peptide-raffinose adducts in (G) Mb-SDA lyophilized with raffinose and (H) Mb-SDA solution with raffinose formulations. Adducts were mapped irrespective of the number of water or raffinose molecules linked. The α-helices from N-terminus to C-terminus in Mb are represented by cylinders labeled A to H respectively. The molecular mass of tryptic fragments 63–77 and 64–78; 63–78 and 64–79 are identical and cannot be differentiated.