Table 2.
Total number of peptide-peptide, peptide-water and peptide-excipient adducts detected by LC-MS in solid- and solution-state Mb-SDA formulations without excipients, with raffinose and with Gdn HCl. The numbers represent the average number of adducts (± SD) from three LC-MS injections.
Type of Adducts | Number of Adducts Detected | |||||
---|---|---|---|---|---|---|
Mb-SDA | Mb-SDA + Raffinose | Mb-SDA + Gdn HCl | ||||
Solid | Solution | Solid | Solution | Solid | Solution | |
Peptide-Peptide | 44.7 ± 3.5 | 30.3 ± 0.6 | 50.7 ± 3.2 | 31.3 ± 1.2 | 105.0 ± 12.3 | 28.3 ± 2.1 |
Peptide-Water | 42.7 ± 3.8 | 30.0 ± 1.7 | 11.3 ± 1.2 | 19.7 ± 1.2 | 74.3 ± 10.2 | 34.3 ± 1.5 |
Peptide-Excipient | N/A | N/A | 11.3 ± 0.6 | 41.3 ± 0.6 | N/A a | N/A a |
Peptide-excipient adducts for the Gdn HCl formulation could not be identified unambiguously by LC-MS and are not reported.