Table 3.
Gingipain activity comparison between different strains at both exponential growth phase and stationary phase. The number showed are percentage to that of the wild-type W83, the data are the average of three biological replicates with three technical replicates each.
| Strain | Genotype | Gingipain activity (%)
|
|||
|---|---|---|---|---|---|
| Exponential phase | Stationary phase | ||||
| Rgp | Kgp | Rgp | Kgp | ||
| W83 | Wild-type | 100 | 100 | 100 | 100 |
| FLL92 | vimA::ermF | 9 | 15 | 29 | 60 |
| FLL370 | ΔrgpB::ermF | 60 | 86 | 56 | 100 |
| FLL371 | ΔrgpB::tetQ-vimA::ermF | 1 | 3 | 7 | 1 |
| FLL372 | ΔrgpA::ermF | 40 | 100 | 39 | 100 |
| FLL373 | ΔrgpA::tetQ-vimA::ermF | 1 | 3 | 31 | 53 |
| FLL374 | Δkgp::ermF | 100 | 0 | 97 | 2 |
| FLL375 | Δkgp::tetQ-vimA::ermF | 2 | 2 | 0 | 2 |
| FLL376 | ΔrgpA::ermF- ΔrgpB::tetQ | 2 | 100 | 2 | 100 |
| FLL377 | ΔrgpA::tetQ- Δkgp::ermF | 46 | 2 | 53 | 1 |
| FLL378 | ΔrgpB::tetQ- Δkgp::ermF | 47 | 0 | 47 | 0 |
| FLL379 | ΔrgpA::cat- Δkgp::tetQ-vimA::ermF | 4 | 3 | 3 | 3 |
| FLL380 | ΔrgpB::cat- Δkgp::tetQ-vimA::ermF | 2 | 4 | 2 | 4 |
| FLL381 | ΔrgpA::cat- ΔrgpB::tetQ-vimA::ermF | 3 | 6 | 5 | 24 |