Table 4.
Monosaccharide composition of W83 RgpB catalytic domain (CD), FLL92 RgpB CD and FLL92 RgpB proenzyme (Unit: mol%). Glycosyl composition of RgpB CD from W83 during exponential phase, RgpB CD from FLL92 during stationary phase, and the RgpB proenzyme from FLL92 during late exponential phase for GC-MS analysis of TMS-methyl glycosides. Experiment was done three times, table is representative of typical data obtained.
| Source | Rib | Fuc | Man | Gal | Glc | GalNAc | GlcNAc |
|---|---|---|---|---|---|---|---|
| W83 RgpB-CD | 7.07 | 0 | 70.42 | 7.66 | 13.64 | 0.35 | 0.86 |
| FLL92 RgpB-CD | 9.40 | 1.12 | 61.33 | 5.97 | 16.70 | 1.92 | 3.55 |
| FLL92 proenzyme | 14.50 | 0 | 69.18 | 2.06 | 14.27 | 0 | 0 |