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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 Mar 9;34(49):5997–6006. doi: 10.1038/onc.2015.48

Figure 5. Stem cell functionality is compromised after miR-200c induction.

Figure 5

(A) There was a significant decrease in the stem cell markers Ezh2 and Bmi1 by qPCR post-DOX treatment. (B) Conversely, the mRNA levels of the luminal progenitor marker Elf5 and the mature luminal marker Gata3 were increased in RFP+ cells, compared with GFP+ cells. (C) A representative FACS profile for CD24+/CD29+ cells shows a change in the overall profile of miR-200c tumors, with the number of double-positive cells decreased relative to untreated controls. (D) Table depicts the results from the limiting dilution transplantation assay. As decreasing numbers of either GFP+ or RFP+ sorted cells were transplanted back into the recipient mouse, fewer tumors arose in each transplantation group. The RFP+ miR-200c-expressing cells showed a significantly reduced CSC frequency, as shown in the bottom row, confirming that miR-200c leads to a reduction in stem cell functionality.