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Overall scheme of purification and activity of pectinase from Aspergillus niger strain MCAS2
| Phase | Volume (ml) | Total activity (U) | Protein (mg/ml) | Protein total (mg) | Specific activity (U/mg) | Yield (%) | Purification fold (X) |
|---|---|---|---|---|---|---|---|
| Crude extract | 100 | 370 | 0.525 | 52.5 | 7.05 | 100 | 1 |
| Acetone ppt. | 7 | 70 | 1.2 | 8.4 | 8.33 | 19 | 1.2 |
| Sephadex G-75 | 3 | 9 | 0.05 | 0.15 | 60 | 16 | 8.5 |
