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. 2015 Aug 24;6:353–371. doi: 10.1016/j.redox.2015.07.017

Fig. 5.

Fig. 5

NO/peroxynitrite signaling is mediated by hydroxyl radicals and not by the carbonate radical 9000 MKN-45 human gastric carcinoma cells per assay received no inhibitor (“control”) or the following inhibitors or scavengers: (A) 25 µM of the peroxynitrite decomposition catalyst FeTPPS, 2.4 mM of the NOS inhibitor l-NAME, 50 mM of the HOCl scavenger taurine (TAU), 100 µM of the NOX inhibitor AEBSF; (B) 20 mM of the carbonate radical scavenger tryptophan (TRYP), 20 mM of the hydroxl radical scavenger mannitol (MANN); (C) 25 µM FeTPPS or the indicated concentrations of mannitol (MANN). 3-AT was added at the indicated concentrations and the percentages of apoptotic cells were determined after 5.5 h. Statistical analysis: Apoptosis induction by 3.1 – 100 mM 3-AT was highly significant (p<0.001). Inhibition of apoptosis by AEBSF and mannitol (between 3 and 100 mM 3-AT), by taurine (25–100 mM) and by l-NAME and FeTPPS (3.1 and 6.2 mM 3-AT) were highly significant (p<0.001), whereas there was no significant inhibitory effect by tryptophan.