Table 1.
Expression analysis of selected genes in VSMCs of Crem−/− and Crem+/+ mice stimulated with 7.5 ng/ml Pdgf-BB by quantitative real-time PCR
| Gene | Relative expression | Standard error | p value |
|---|---|---|---|
| Hprt | 1.054 | ||
| Ywhaz | 0.949 | ||
| Ppia | 1.450 | 1.13–1.87 | 0.004 up |
| Arhgap12 | 1.675 | 1.29–2.24 | 0.006 up |
| Rgs5 | 2.562 | 1.27–4.81 | 0.009 up |
| Pdgfra | 1.568 | 1.09–2.55 | 0.026 up |
| Taf12 | 1.458 | 0.93–2.23 | 0.052 |
| Fgf18 | 1.716 | 0.92–2.81 | 0.054 |
| Agtrap | 5.125 | 0.73–20.88 | 0.062 |
Relative expression ratios, standard error, and statistical analysis were calculated by the relative expression software tool (REST© Version 2.07). An upregulation indicates an elevated amount of mRNA in the Crem−/− VSMCs (n = 5)