Figure 1.

siRNA knockdown of Alix inhibits egress of eVP40 VLPs. A, HEK293T cells were mock-transfected or transfected with eVP40 plus random or Alix-specific siRNAs. eVP40, endogenous Alix, and actin were detected in cell extracts, and eVP40 was detected in VLPs by Western blot. B, Quantification of the relative budding efficiency of eVP40 VLPs from cells receiving random siRNAs (set at 100%) or Alix-specific siRNAs. Each bar represents the average ±SEM of 3 independent experiments. Abbreviations: SEM, standard error of the mean; VLP, virus-like particle.