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. 2015 Sep 1;212(Suppl 2):S138–S145. doi: 10.1093/infdis/jiu838

Figure 3.

Figure 3.

Bro1-V region of Alix rescues budding of eVP40-ΔPT/PY L-domain mutant most efficiently. HEK293T cells were mock-transfected (lane 1) or transfected with eVP40-ΔPT/PY plus empty vector (lane 2), Bro1 domain (lane 3), Bro1-V domain (lane 4), or V-PRD domain (lane 5). eVP40-ΔPT/PY, Bro1, Bro1-V, V-PRD, and actin were detected in cell extracts by Western blot, and eVP40-ΔPT/PY was detected in VLPs by Western blot. Numbers in parentheses represent fold change in levels of VLPs relative to control (lane 2). Abbreviations: VLP, virus-like particle; WT, wild type.