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. 2015 Sep 1;212(Suppl 2):S138–S145. doi: 10.1093/infdis/jiu838

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© The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

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Figure 4. — eVP40-ΔPT/PY interacts with Bro1-V fragment of Alix. A, Total protein from cells transfected with eVP40-ΔPT/PY plus vector alone (lanes 1 and 2) or Bro1-V (lanes 3 and 4) was first immunoprecipitated with either rabbit pre-immune (IgG; lanes 1 and 3) serum, or anti-eVP40 (lanes 2 and 4) antiserum as indicated, and anti-HA antiserum was used to detect HA-tagged Bro1-V in the precipitated samples by Western analysis. B, Controls for expression of eVP40-ΔPT/PY, Bro1-V, and actin, are shown. Abbreviation: VLP, virus-like particle.