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Liposome content release assay and characterization of mutants. A, Release of 8-aminonapthalene-1,3,6 trisulfonic acid and p-xylene-bis-pyridinium bromide contents of wild-type Marburg virus GP2 fusion loop, as well as alanine mutants, at pH 4 and pH 7. B, Circular dichroism spectra of alanine mutant proteins at several pH conditions in 1,2-dimyristoyl-sn-glycero-3-phosphocholine/1,2-dihexanoyl-sn-glycero-3-phosphocholine bicelles at protein concentration of 40 µM.
