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. 2015 Sep 1;212(Suppl 2):S258–S270. doi: 10.1093/infdis/jiv182

Figure 2.

Figure 2.

Histopathology of liver: Panels include Hemotoxylin & Eosin (H&E) MARV-Rav (A, E, I, and M) with corresponding anti-Marburg immunohistochemistry (IHC) (B, F, J, and N), H&E MARV Ang (C, G, K, and O) with corresponding anti-Marburg IHC (D, H, L, and P), and H&E control (Q) which has no significant lesions (NSL), nor any significant immunolabeling in corresponding anti-Marburg IHC (R). All images are 20x with 60x inserts. MARV-Rav-infected GP liver at day 3 (A) had NSL detected but had diffuse cytoplasmic immunolabeling of kupffer cells present (B). H&E of MARV-Rav-infected GP liver on day 5 (E) presented evidence of multifocal hepatocellular degeneration/necrosis and sinusoidal leukocytosis with diffuse cytoplasmic immunolabeling of Kupffer cells and small clusters of hepatocytes (F). Day 7 MARV-Rav-infected GP demonstrated progressive multifocal hepatocellular vacuolar degeneration/necrosis and sinusoidal leukocytosis on H&E (I) which was accompanied by diffuse cytoplasmic immunolabeling of Kupffer cells and clusters of hepatocytes (J). Terminal MARV-Rav-infected GP had advanced multifocal hepatocellular vacuolar degeneration/necrosis and sinusoidal leukocytosis (M) accompanied by diffuse cytoplasmic immunolabeling of Kupffer cells and sheets of hepatocytes (N). Day 3 MARV-Ang-infected GP had NSL on H&E (C) yet diffuse cytoplasmic immunolabeling of Kupffer cells and small clusters of hepatocytes was present (D). Day 5 MARV-Ang-infected GP had multifocal hepatocellular degeneration/necrosis and sinusoidal leukocytosis on H&E (G) which was associated with diffuse cytoplasmic immunolabeling of Kupffer cells and clusters of hepatocytes (H). Day 7 MARV-Ang-infected GP had marked multifocal hepatocellular vacuolar degeneration/necrosis and sinusoidal leukocytosis on H&E (K) with diffuse cytoplasmic immunolabeling of Kupffer cells and clusters of hepatocytes (L). Terminal MARV-Ang-infected GP had severe, multifocal hepatocellular vacuolar degeneration/necrosis and sinusoidal leukocytosis on H&E (O) associated with diffuse cytoplasmic immunolabeling of Kupffer cells and sheets of hepatocytes (P). Abbreviations: GP, guinea pig; MARV, Marburg virus.