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Schematic diagram of immunization and challenge study design. Female Balb/c mice (groups of 5) were vaccinated at 4-week intervals with different immunization regimens. Blood samples were collected 2 weeks after the second and third immunizations, and mice were challenged 12 weeks after the final immunization by 1000 plaque-forming units (PFU) of mouse-adapted Ebola virus (EBOV). Abbreviations: GP, glycoprotein; sGP, truncated Ebola virus glycoprotein isoform.
