Figure 2.

Antibody response induced by different regimens of Ebola virus (EBOV) glycoprotein (GP) and truncated EBOV GP isoform (sGP) DNA vaccines. Groups of mice were vaccinated by intramuscular injection with 50 µg of DNA (25 µg/leg) according to the schedule shown in Figure 1. A, Antibody response against GP. B, Antibody response against sGP. The levels of antibody response induced by EBOV GP DNA constructs in mice were measured by an enzyme-linked immunosorbent assay, using purified histidine-tagged GP or sGP proteins as coating antigen. The antibody concentration was determined from a standard curve. Results are mean values and standard deviations for samples from individual animals of each group. Abbreviation: IgG, immunoglobulin G.