Figure 3.

Neutralization of Ebola virus (EBOV) glycoprotein (GP)–mediated pseudovirion infection. Neutralizing activity of immune sera collected after the third immunization was determined by incubating 500 plaque-forming units (PFU) of GP-pseudotyped virus with a 1:900 dilution of serum samples from mice that received different immunization regimens, shown in Figure 1. Neutralization was measured as the decrease in luciferase expression, compared with virus-only controls, after 48 hours, as described in “Materials and Methods” section. Results reported are means and standard deviations for samples from individual animals of each group.