Skip to main content
. 2015 Sep 1;212(Suppl 2):S359–S367. doi: 10.1093/infdis/jiv353

Figure 2.

Figure 2.

Escherichia coli–produced recombinant VP40 and Ebola virus (EBOV) virus-like particles (VLPs) precipitated from supernatants of HEK-293T/17 cell cultures transfected with EBOV VP40, EBOV nucleoprotein (NP), and EBOV glycoprotein (GP) mammalian expression constructs were resolved on nonreducing Bis-Tris/MES sodium dodecyl sulfate polyacrylamide gels. Proteins were transferred to nitrocellulose membranes and probed with goat anti-VP40 or goat anti-NP polyclonal antibodies (VHFC) and a rabbit anti-goat immunoglobulin G (H + L)-HRP (KPL). Blots were imaged with LumiGlo chemiluminescent substrate (KPL) in a GE ImageQuant LAS4000 imager.