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. 2015 Aug 4;5(8):e612. doi: 10.1038/tp.2015.102

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Copyright © 2015 Macmillan Publishers Limited

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α1, α2, β1 and γ2 GABA_AR subunit expression are not different between diagnostic groups in the total homogenate, ER or SYN fractions. Western blot analysis of α1, α2_ALL, β1 and γ2 GABA_AR subunit expression in schizophrenia and comparison subjects. There are no differences between diagnostic groups in the protein expression of α1, α2_ALL, β1 or γ2 GABA_AR subunits in (a) the total homogenates, (b) ER fractions or (c) the SYN fractions. Data are expressed as the mean signal intensity (±s.e.m.) of protein targets in the ER fraction normalized to VCP as a loading control, and JM4 as an ER marker or gephyrin as an inhibitory synapse marker, relative to the VCP-normalized signal intensity of the same target in the total homogenate. COMP, comparison subject; ER, endoplasmic reticulum; GABA_AR, γ-aminobutyric acid type A receptor; SCZ, schizophrenia; SYN, synapse; VCP, valosin-containing protein.