Figure 4.

Comparison of the different algorithms for determining the total intensity of a focus. (A and B) The ratio of the signal in the focus (as estimated utilizing the different algorithms) versus the known total cellular signal plotted for the two different strains DnaN (A) and DnaQ (B), respectively. Here we plot the percentage of the estimated focus content divided by the known total signal. (Inset) The resulting error in terms of the difference between the focus content from the fit and the known focus content divided by the known focus content, plotted as function of FCR. We only show the fitted results up and until 100%. n = 42 cells for both (A) and (B). To see this figure in color, go online.