Table 1.
Lag times between the onset of leakage of each dye and the time interval before the initial leakage takes place after the addition of peptide
Time Delays (s) | PC/PE | PC/PE/PS | PC/PS | PC |
---|---|---|---|---|
t0 − tCF | 1600 ± 110 | 760 ± 120 | 1400 ± 60 | 1600 |
tCF – t3k-CB | 1.8 ± 0.6 | 1.5 ± 0.3 | 41 ± 5a | 160 ± 110 |
tCF – t10k-AF647 | 4.2 ± 1.5 | 2.0 ± 0.4 | 52 ± 6 | 220 ± 66 |
t3k-CB – t10k-AF647 | 2.7 ± 0.9 | 1.4 ± 0.4 | 9.6 ± 1.2 | 60 ± 42 |
The errors represent the standard deviation of the observed GUV data set.
For PC/PS GUVs, the tCF – t3k-CB data only includes GUVs that leaked to all three dyes; these sample conditions contained two distinct populations of GUV leakage behaviors where a second population of GUVs only leaked to the CF and 3k-CB dyes with a time delay of tCF – t3k-CB = 4.8 ± 0.6 s.