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. 2015 Sep 10;6:901. doi: 10.3389/fmicb.2015.00901

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Copyright © 2015 Gulmann, Beaulieu, Shank, Ding, Seyfried and Sievert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gammaproteobacteria phylogenetic tree. Neighbor-joining trees were constructed from aligned, full-length clone 16S rRNA sequences of OTUs detected more than once, based on 1,153 homologous positions filtered by base frequency (50%) using MEGA6. Tag sequences that were identical to a clone sequence were identified. Bootstrap values were calculated in MEGA6, and nodes with >50% support, based on 1,000 replicates are displayed. Haliangium tepidum (NR_024781) and Kofleria flava (NR_041981) were used as outgroups.