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. 2015 Sep 10;5:13878. doi: 10.1038/srep13878

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Photographs of 30-day-old gene-modified lambs (Photo taken by X.W.). (b) PCR products of the targeted region of MSTN and FGF5 from founder goats co-microinjected with a mixture of Cas9 mRNA and sgRNAs. (c) Detection of sgRNA:Cas9-mediated on-target cleavage of MSTN and FGF5 by T7E1 cleavage assay. All PCR products from (b) were subjected to T7E1 cleavage assay. All the samples were digested by T7E1, suggesting that all founders carry MSTN and FGF5 mutations. (d) Sequencing results of modified MSTN and FGF5 loci detected in lambs.