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. 2015 Sep 10;5:13878. doi: 10.1038/srep13878

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(a) PCR products of the potential off-target sites of MSTN and FGF5 sgRNA:Cas9 from founder lambs. A total of 13 potential off-target sites most homologous to MSTN and FGF5 sgRNA were named OT1 to OT13. OT5 and OT7 were selected and PCR amplified from genomic DNA from founders. (b) Detection of sgRNA:Cas9-mediated off-target cleavage of MSTN and FGF5 by T7E1 cleavage assay. All PCR products from (a) were subjected to T7E1 cleavage assay. (c) Sequencing results of PCR products.