Table 1. Summary of production of gene-modified goats via CRISPR-Cas9.
Goats forzygotecollection | Collectedembryos | Cas9-sgRNA injectedembryos (one-cell stage) |
Recipientgoatsa | Newborns | Alive goats | Gene-modified goats | |
---|---|---|---|---|---|---|---|
Injectedembryos | Transferredembryos | ||||||
79 | 926 | 862 | 416 | 137 | 93 | 79 | 26 |
aFive surrogate ewes had aborted.