Figure 3. Proton radiation alters Chk1/2 phosphorylation and shortens G2 arrest recovery.

IN528 GSCs were irradiated with 10 Gy of proton beam or x-ray photon radiation. (A) Cell lysates were immunoblotted with anti-P-Chk1-Ser³¹⁷, anti-P-Chk2-Thr⁶⁸, and anti-GAPDH antibodies. Full-length blots are presented in Supplementary Figure 1. (B,C) Cell Cycle was analyzed by flow cytometry (means ± SEM, n = 4–8). p values were determined by Student’s t tests. (B) Proton radiation (C) Photon radiation.