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. 2015 Sep 10;5:13961. doi: 10.1038/srep13961

Figure 5. Proton radiation induces ROS-dependent cytotoxicity.

Figure 5

IN528 GSCs were irradiated with proton beam or x-ray photon at different doses. (A) Intracellular ROS levels in GSCs were measured three days after irradiation by flow cytometry. Left, representative data. Right, Quantification of ROS level three days post irradiation (means ± SEM, n = 4–8). (B,C) Cells were treated with TEMPOL (10 mM) one hour prior to proton or photon irradiation. (B) ROS levels were determined by flow cytometry (means ± SEM, n = 9–10). (C) Cells were subjected to clonogenic survival analysis (means ± SEM, n = 5). p values were determined by Student’s t tests.