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. 2015 Sep 10;5:13916. doi: 10.1038/srep13916

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(a) Caspase-3 immunostaining (green) reveals spread apoptotic fibers or group of dying cells in both dy^3K/dy^3K and dy^3K/itga7 muscles. Collagen IV antibody (red staining) and DAPI (blue) were used to delineate muscle fibers and show nuclei. (b) CD11b staining (red) showed infiltration of macrophages into dy^3K/dy^3K and dy^3K/itga7 dystrophic triceps muscle. DAPI (blue) depicts cell nuclei. (c) Percentage of apoptotic fibers was not increased in double knockout triceps muscle compared to dy^3K/dy^3K triceps (p = 0.6828, Mann-Whitney test). (d) Amount of macrophages was not significantly different between dy^3K/dy^3K and dy^3K/itga7 triceps (p = 0.7143, Mann-Whitney test). The numbers of animals used are indicated in the graph. Scale bar, 50 μm.