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. 2015 Sep 1;109(5):892–899. doi: 10.1016/j.bpj.2015.07.039

Figure 1.

Figure 1

Mitochondrial donut formation and diameter measurements. (A) A typical event of donut formation. The mitochondrion was tubular at 0 s, then bent to self-fusion at 50 s and finally formed a donut shape at 64 s. Scale bar, 1 μm. (B) Donut diameter was measured with images taken with a confocal microscope. The mitochondria were marked with mtGFP. The fluorescence profile along the red line (left) is shown in the middle image. The distribution of measured diameters is shown at right, and the red line shows the Gaussian fit (n = 111). Scale bar, 1 μm. (C) STORM imaging of mitochondria in MEF cells. Mitochondria were marked with TOMM20 antibody, and the image was reconstructed from 100,000 images. Scale bar, 0.2 μm. (D) 3D PALM imaging of mitochondria. The parallel lines were used to measure the diameter. The color (green-red) indicates the z-axis depth of the structure. Scale bar, 0.2 μm. (E) TEM imaging of mitochondria. The arrow shows the diameter. (F) Distributions of mitochondrial diameters measured by STORM (n = 55), PALM (n = 33), and TEM (n = 57). ∗∗∗p < 0.001 using the two-tailed t-test.