Figure 6.

Differences in the interhelical interaction due to the mutation A86L^1.54. (A) The direct and indirect contacts of N82^1.50 with A110^2.47 and S362^7.46 in the vicinity of the A86L^1.54 mutation in NTSR1-GW5 (B) and the wild type receptor. Two waters that have important roles in indirect contacts between N82^1.50 and S362^7.46 were observed in 53 and 83% of the snapshots from MD simulation trajectories, respectively. (C and D) Hydrophobic interaction pattern near the A86L^1.54 mutation in NTSR1-GW5 and wild type receptor. The T79^1.47–L114^2.51 and A86L^1.54–L114^2.51 interactions in NTSR1-GW5 and T85^1.53–A110^2.47 hydrophobic interaction in the wild type receptor are highlighted by orange double arrows. The van der Waals surfaces of the atoms are shown as mesh, and the distances shown in orange are in Å.