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. 2015 Sep 10;5:13995. doi: 10.1038/srep13995

Figure 3. Mice infected with γHV-68 AC-RTA have very low CD8 T cell infiltrations and mount the same type of T helper response inside the CNS as naïve EAE mice.

Figure 3

Mice were infected with γHV-68 (black bars) or γHV-68 AC-RTA (grey bars) or MEM only (open bars). Five weeks p.i. EAE was induced. Two weeks post EAE induction, mice were perfused, brain and spinal cords were harvested and immune infiltrations were isolated. Isolated immune cells were stimulated with PMA and ionomycin and stained for CD4 (A) and CD8 (A) to measure IFN-γ and IL-17 (B) production. The amount of CD8 T cells infiltrating inside the CNS of γHV-68 AC-RTA EAE and naïve EAE mice was too low to perform intracellular staining (B). Three separate experiments with 5–6 mice/group; data are represented as mean, error bars are SEM and were analyzed with t-test: **p < 0.01; ***p < 0.001.