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Design and optimization of the α7-nAChR expression construct. (A) Schematic depiction of the N-terminal double-MBP expression construct. (B–F) Western blot analysis of α7 nAChRs from 10 different species (B), N-terminally truncated α7 nAChR (C), C-terminally truncated α7 nAChR (D), glycosylation of wild-type (N+) and mutated (N–) receptor (E), and the receptor with a GSA-linker replacement of the intracellular loop (F).
