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Large-scale expression and purification of the WT α7-nAChR. (A) Size-column purification of the MBP-tagged (before TEV cleavage) Taeniopygia guttata and untagged (after TEV cleavage) α7-nAChR. (B) SDS-PAGE analysis of the elution fractions from the amylose column (lane 1), the sample digested with TEV protease (lane 2), the pooled fractions from size-exclusion chromatography of the TEV-digested sample (lane 3), the double-MBP tag (lane 4), and TEV protease (lane 5).
