Figure 4.

Expression of Total PDGFRB Receptor in HeLa Cells Transfected with WT, p.Leu658Pro, and p.Val665Ala PDGFRB Constructs

Representative western blots showing total PDGFRB (A), phosphorylated PDGFRB at tyrosine 751 (p-Tyr751) (B), and tyrosine 1021 (p-Tyr1021) (C) of the WT (wild-type), p.Leu658Pro, and p.Val665Ala variants. α-tubulin is shown as a protein loading control (D). Horizontal bars indicate the electrophoretic mobility of three mature forms of PDGFRB (180, 160, and 130 kDa) (E). Quantitative analysis of the three mature forms of total PDGFRB was observed by western blot (n = 3). Values represent the percentage (average ± S.E.M.) of each PDGFRB form from the total PDGFRB in WT cells. ^∗∗∗ = p < 0.001, ^∗∗∗∗ = p < 0.0001. ns, non-significant.